Defeat Blue Viper boats without taking significant damage.
― In-game description[src]
"Canal Chase - Expert Course" is the first Master Thief Challenge in "An Opera of Fear" of Sly 3: Honor Among Thieves, and it is the first challenge in the game overall. It is a variation of the "Canal Chase" mission, with the variation being that Sly and Bentley's boat has half of the amount of health available. Also, the bullets do a little more damage to the Blue Vipers, while objects in the canal do little damage to you.
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Sly 3 Honor Among Thieves - Master Thief Challenge - Canal Chase (Expert Course)